Vascular differentiation of multipotent spermatogonial stem cells derived from neonatal mouse testis

DOI: 10.3858/emm.2012.44.4.034

Authors: Ji-Eun Im; Sun-Hwa Song; Ji Yeon Kim; Koung Li Kim; Sang Hong Baek; Dong Ryul Lee; Wonhee Suh

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” mouse mSSC in “unspecified medium” for “2” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should be changed once in “2” days.

Ideally, “mSSC” should express “POU5F1”, “NANOG”, “PECAM1” at the end of culture.

Step 2: mesoderm 1

day 2 ~ 6

culture “spheroid” mouse mSSC in “DMEM/F12 medium” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “mesoderm” should express “TBXT”, “KDR”, “MESP1”, “Islet1”, “Nkx2.5” at the end of culture.

Step 3: endothelial 1

day 6 ~ 16

culture “adherent” mouse mSSC in “unspecified medium” for “10” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “10” days.

Ideally, “EC” should express “CDH5”, “PECAM1” at the end of culture.