Exosomes secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells attenuate limb ischemia by promoting angiogenesis in mice

DOI: 10.1186/scrt546

Authors: Guo-wen Hu; Qing Li; Xin Niu; Bin Hu; Juan Liu; Shu-min Zhou; Shang-chun Guo; Hai-li Lang; Chang-qing Zhang; Yang Wang; Zhi-feng Deng

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.