Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip

DOI: 10.1186/s40580-021-00285-4

Authors: Han Na Lee; Yoon Young Choi; Jin Won Kim; Young Seo Lee; Ji Wook Choi; Taewook Kang; Yong Kyun Kim; Bong Guen Chung

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “3” “%”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 3

culture “adherent” human PSC in “RPMI” for “2” days. For ECM, use “Matrigel” at “3” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 3: mesoderm 1

day 3 ~ 5

culture “adherent” human PSC in “RPMI” for “2” days. For ECM, use “Matrigel” at “3” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 4: endothelial 1

day 5 ~ 11

culture “device” human PSC in “RPMI 1640” for “6” days. For ECM, use “Matrigel” at “1.5” “%”. Medium should contain factors shown in the following table, and should be changed once in “6” days.

Ideally, “EC” should express “PECAM1” at the end of culture.