Motor neurons and endothelial cells additively promote development and fusion of human iPSC-derived skeletal myocytes

DOI: 10.1186/s13395-024-00336-4

Authors: Suradip Das; Melanie C. Hilman; Feikun Yang; Foteini Mourkioti; Wenli Yang; D. Kacy Cullen

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “StemMACS iPS-Brew medium” for “1” days. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.