BCL6B-dependent suppression of ETV2 hampers endothelial cell differentiation

DOI: 10.1186/s13287-024-03832-y

Authors: Zhonghao Li; Wei Wu; Qiushi Li; Xin Heng; Wei Zhang; Yinghong Zhu; Lin Chen; Ziqi Chen; Mengcheng Shen; Ning Ma; Qingzhong Xiao; Yi Yan

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. “PSC” should be “upregulated” for “BCL6B”, “downregulated” for “BCL6B”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2

culture “adherent” human PSC in “E8” for “1” days. “PSC” should be “upregulated” for “BCL6B”, “downregulated” for “BCL6B”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 3: mesoderm 1

day 2 ~ 5

culture “adherent” human PSC in “IMDM” for “3” days. “PSC” should be “upregulated” for “BCL6B”, “downregulated” for “BCL6B”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 4: endothelial 1

day 5 ~ 7

culture “adherent” human PSC in “IMDM” for “2” days. “PSC” should be “upregulated” for “BCL6B”, “downregulated” for “BCL6B”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should be obtained at the end of culture. Ideally, “EC” should be obtained at the end of culture.