Exosomes secreted by endothelial cells derived from human induced pluripotent stem cells improve recovery from myocardial infarction in mice
DOI: 10.1186/s13287-023-03462-w
Authors: Hao Li; Lu Wang; Teng Ma; Zhongmin Liu; Ling Gao
Step 1: iPSC/ESC maintainance
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “unspecified method” human PSC in “unspecified medium”.
Ideally, “PSC” should be obtained at the end of culture.
Step 2: endothelial 1
day 1 ~ 2 (duration missing in database; assumed 1 day)
culture “unspecified method” human PSC in “unspecified medium”.
Ideally, “EC” should express “PECAM1”, “CDH5”, “VWF” at the end of culture.