Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signaling

DOI: 10.1186/s13287-023-03235-5

Authors: Hyo Jin Kim; Gyeongmin Kim; Kyun Yoo Chi; Hyemin Kim; Yu Jin Jang; Seongyea Jo; Jihun Lee; Youngseok Lee; Dong-Hun Woo; Choongseong Han; Sang Kyum Kim; Han-Jin Park; Jong-Hoon Kim

Step 1: Primitive streak 1

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

Ideally, “PS” should express “TBXT”, “MIXL1” at the end of culture.

Step 2: endothelial 1

day 1 ~ 7

culture “unspecified method” unknown species cell in “Stemline II medium” for “6” days. Medium should contain factors shown in the following table, and should be changed once in “6” days.

Ideally, “EC” should express “PECAM1”, “VECAD”, “VWF”, “PECAM1” at the end of culture.

Step 3: endothelial 2

day 7 ~ 23

culture “spheroid” unknown species cell in “unspecified medium” for “16” days. Medium should contain factors shown in the following table, and should be changed once in “16” days.

Ideally, “EC” should express “PECAM1”, “KDR” at the end of culture.