Enhanced HSC-like cell generation from mouse pluripotent stem cells in a 3D induction system cocultured with stromal cells

DOI: 10.1186/s13287-021-02434-2

Authors: Wei Shan; Qin Yu; Yan Long; Qian Luo; Honghu Li; Yingli Han; Yulin Xu; Shan Fu; Xiangjun Zeng; Cong Wei; Yang Gao; Xiaoqing Li; Xia Li; Lifei Zhang; Lizhen Liu; Ming Chen; Pengxu Qian; He Huang

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse ESC in “DMEM/F12”. For ECM, use “Fibroblast” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “ESC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 7

culture “spheroid” unknown species cell and mouse ESC in “unspecified medium” for “6” days. Medium should be changed once in “6” days.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 3: endothelial 1

day 7 ~ 12

culture “adherent” unknown species cell and mouse ESC in “alpha-MEM” for “5” days. For ECM, use “Gelatin” at “0.1” “%”. Medium should contain factors shown in the following table, and should be changed once in “5” days.

Ideally, “EC” should be obtained at the end of culture. Ideally, “HE” should be obtained at the end of culture. Ideally, “LSK” should be obtained at the end of culture.