Pluripotent stem cell-induced skeletal muscle progenitor cells with givinostat promote myoangiogenesis and restore dystrophin in injured Duchenne dystrophic muscle

DOI: 10.1186/s13287-021-02174-3

Authors: Wanling Xuan; Mahmood Khan; Muhammad Ashraf

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 2: mesoderm 1

day 1 ~ 3

culture “adherent” human PSC in “E6 medium” for “2” days. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 3: mesoderm 2

day 3 ~ 8

culture “adherent” human PSC in “E6 medium” for “5” days. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “5” days.

Ideally, “MPC” should express “PAX7”, “DES”, “ITGA4”, “RAC2”, “FGF”, “ENAH”, “IL6” at the end of culture.