Transplantation of retinal pigment epithelium and photoreceptors generated concomitantly via small molecule-mediated differentiation rescues visual function in rodent models of retinal degeneration

DOI: 10.1186/s13287-021-02134-x

Authors: Harshini Surendran; Swapna Nandakumar; Vijay Bhaskar Reddy K; Jonathan Stoddard; Varsha Mohan K; Pramod K. Upadhyay; Trevor J. McGill; Rajarshi Pal

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “1” “%”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.