MSX2 suppression through inhibition of TGFβ signaling enhances hematopoietic differentiation of human embryonic stem cells

DOI: 10.1186/s13287-020-01653-3

Authors: Hongtao Wang; Mengge Wang; Yu Wang; Yuqi Wen; Xiaoyuan Chen; Dan Wu; Pei Su; Wen Zhou; Lihong Shi; Jiaxi Zhou

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “ESC” should express “NANOG”, “SOX2”, “POU5F1” at the end of culture.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. “ESC” should be “upregulated” for “MSX2”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “ME” should express “APLNR” at the end of culture.

Step 3: endothelial 1

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. “ESC” should be “downregulated” for “MSX2”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “EC” should express “PECAM1” at the end of culture.