Induced pluripotent stem cell-derived endothelial progenitor cells attenuate ischemic acute kidney injury and cardiac dysfunction

DOI: 10.1186/s13287-018-1092-x

Authors: Wen-Ching Shen; Yu-Hsiang Chou; Hsiang-Po Huang; Jenn-Feng Sheen; Shih-Chieh Hung; Hsin-Fu Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. “PSC” should be “upregulated” for “GFP”. For ECM, use “Fibroblast” at “N/A” “N/A”.

Ideally, “PSC” should express “CD133” at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

Ideally, “EPC” should express “CD133”, “KDR” at the end of culture.