Human liver organoids generated with single donor-derived multiple cells rescue mice from acute liver failure

DOI: 10.1186/s13287-017-0749-1

Authors: Yun-Zhong Nie; Yun-Wen Zheng; Miyuki Ogawa; Etsuko Miyagi; Hideki Taniguchi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should express “POU5F1”, “NANOG”, “SOX2”, “LIN28A”, “KLF4”, “SSEA4”, “TRA-1-60” at the end of culture.