Human iPS cell-derived mural cells as an in vitro model of hereditary cerebral small vessel disease

DOI: 10.1186/s13041-020-00573-w

Authors: Yumi Yamamoto; Katsutoshi Kojima; Daisuke Taura; Masakatsu Sone; Kazuo Washida; Naohiro Egawa; Takayuki Kondo; Eiko N. Minakawa; Kayoko Tsukita; Takako Enami; Hidekazu Tomimoto; Toshiki Mizuno; Raj N. Kalaria; Nobuya Inagaki; Ryosuke Takahashi; Mariko Harada-Shiba; Masafumi Ihara; Haruhisa Inoue

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “Primate ES Cell Medium”. For ECM, use “SNL feeder” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 4

culture “adherent” human PSC in “DMEM/F12” for “3” days. For ECM, use “Collagen” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Step 3: endothelial 1

day 4 ~ 8

culture “adherent” human PSC in “StemPro” for “4” days. For ECM, use “Collagen” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “EC” should be obtained at the end of culture.