Induced pluripotent stem cell-derived cells model brain microvascular endothelial cell glucose metabolism

DOI: 10.1186/s12987-022-00395-z

Authors: Callie M. Weber; Bilal Moiz; Sophia M. Zic; Viviana Alpízar Vargas; Andrew Li; Alisa Morss Clyne

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR-Plus”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2

culture “adherent” human PSC in “E6 medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Step 3: endothelial 2

day 2 ~ 4

culture “adherent” human PSC in “hESFM” for “2” days. For ECM, use “Collagen” at “0.4” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should be obtained at the end of culture.