Commentary on human pluripotent stem cell-based blood–brain barrier models
DOI: 10.1186/s12987-020-00222-3
Authors: Ethan S. Lippmann; Samira M. Azarin; Sean P. Palecek; Eric V. Shusta
Step 1: endothelial 1
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “unspecified method” unknown species cell and human PSC in “unspecified medium”. Medium should contain factors shown in the following table.
| concentration | unit | |
|---|---|---|
| retinoic acid | — | — |
Ideally, “EC” should express “PECAM1”, “CDH5”, “VWF”, “KDR”, “TEK”, “SOX17” at the end of culture.