Chemically defined human vascular laminins for biologically relevant culture of hiPSC-derived brain microvascular endothelial cells

DOI: 10.1186/s12987-020-00215-2

Authors: Pedram Motallebnejad; Samira M. Azarin

Step 1: endothelial 1

day 0 ~ 1

culture “adherent” human PSC in “human endothelial serum-free medium” for “1” days. For ECM, use “laminin 511-E8” at “0.01” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “EC” should be obtained at the end of culture.

Step 2: endothelial 2

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “human endothelial serum-free medium”. For ECM, use “laminin 511-E8” at “0.01” “g/m2”. Medium should contain factors shown in the following table.

Ideally, “EC” should be obtained at the end of culture.