Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood–brain barrier model
DOI: 10.1186/s12987-019-0147-4
Authors: Louise Delsing; Therése Kallur; Henrik Zetterberg; Ryan Hicks; Jane Synnergren
Step 1: endothelial 1
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “unspecified method” unknown species cell and human EC in “unspecified medium”. Medium should contain factors shown in the following table.
| concentration | unit | |
|---|---|---|
| serum | — | — |
Ideally, “EC” should be obtained at the end of culture.