Modeling and rescue of defective blood–brain barrier function of induced brain microvascular endothelial cells from childhood cerebral adrenoleukodystrophy patients
DOI: 10.1186/s12987-018-0094-5
Authors: Catherine A. A. Lee; Hannah S. Seo; Anibal G. Armien; Frank S. Bates; Jakub Tolar; Samira M. Azarin
Step 1: iPSC/ESC maintainance
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”.
Ideally, “PSC” should be obtained at the end of culture.
Step 2: endothelial 1
day 1 ~ 3
culture “adherent” human PSC in “Human Endothelial-SFM” for “2” days. Medium should contain factors shown in the following table, and should be changed once in “2” days.
| concentration | unit | |
|---|---|---|
| platelet-poor plasma derived serum | 1 | % |
Ideally, “EC” should express “PECAM1”, “CDH5”, “claudin-5”, “occludin”, “P-glycoprotein”, “SLC2A1” at the end of culture.