Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making

DOI: 10.1186/s12885-023-11078-9

Authors: Swati Chitrangi; Pooja Vaity; Aishwarya Jamdar; Shweta Bhatt

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should express “POU5F1”, “NANOG”, “SOX2”, “SSEA4”, “TRA-1-81”, “TRA-1-60”, “ALP” at the end of culture.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “N2B27 medium”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Step 3: endothelial 1

day 2 ~ 5

culture “adherent” human PSC in “StemPro-34 medium” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “EC” should express “PECAM1” at the end of culture.