Genomic chart guiding embryonic stem cell cardiopoiesis
Authors: Randolph S Faustino; Atta Behfar; Carmen Perez-Terzic; Andre Terzic
Step 1: iPSC/ESC maintainance
day 0 ~ 2
culture “unspecified method” mouse ESC in “GMEM” for “2” days. Medium should contain factors shown in the following table, and should be changed once in “2” days.
| concentration | unit | |
|---|---|---|
| FBS | 7.5 | % |
Ideally, “ESC” should be obtained at the end of culture.