Possible linkages between the inner and outer cellular states of human induced pluripotent stem cells

DOI: 10.1186/1752-0509-5-S1-S17

Authors: Shigeru Saito; Yasuko Onuma; Yuzuru Ito; Hiroaki Tateno; Masashi Toyoda; Akutsu Hidenori; Koichiro Nishino; Emi Chikazawa; Yoshihiro Fukawatase; Yoshitaka Miyagawa; Hajime Okita; Nobutaka Kiyokawa; Yohichi Shimma; Akihiro Umezawa; Jun Hirabayashi; Katsuhisa Horimoto; Makoto Asashima

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “iPSellon medium”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.