Characterization of in vivo chemoresistant human hepatocellular carcinoma cells with transendothelial differentiation capacities
Authors: Christian Marfels; Miriam Hoehn; Ernst Wagner; Michael Günther
Step 1: endothelial 1
day 0 ~ 42
culture “unspecified method” unknown species cell in “unspecified medium” for “42” days. For ECM, use “agarose” at “N/A” “N/A”. Medium should be changed once in “42” days.
Step 2: endothelial 2
day 42 ~ 43
culture “unspecified method” unknown species cell in “unspecified medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.
Ideally, “EC” should express “ICAM2”, “PECAM1” at the end of culture.