Generation and validation of a PITX2–EGFP reporter line of human induced pluripotent stem cells enables isolation of periocular mesenchymal cells
Authors: Toru Okubo; Ryuhei Hayashi; Shun Shibata; Yuji Kudo; Yuki Ishikawa; Saki Inoue; Yuki Kobayashi; Ai Honda; Yoichi Honma; Satoshi Kawasaki; Kohji Nishida
Step 1: iPSC/ESC maintainance
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “adherent” human PSC in “StemFit medium”. For ECM, use “Laminin” at “0.005” “g/m2”.
Ideally, “PSC” should express “NANOG”, “OCT3/4”, “TRA-1-60”, “SSEA-4” at the end of culture.