Cornea organoids from human induced pluripotent stem cells
DOI: 10.1038/srep41286
Authors: James W. Foster; Karl Wahlin; Sheila M. Adams; David E. Birk; Donald J. Zack; Shukti Chakravarti
Step 1: iPSC/ESC maintainance
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “spheroid” human PSC in “mTeSR”. Medium should contain factors shown in the following table.
| concentration | unit | |
|---|---|---|
| blebbistatin | 5.0e-6 | mol/l |
Ideally, “PSC” should be obtained at the end of culture.