Role of Mir-155 in Controlling HIF-1α Level and Promoting Endothelial Cell Maturation

DOI: 10.1038/srep35316

Authors: Deguang Yang; Jinhong Wang; Meng Xiao; Tao Zhou; Xu Shi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “DMEM/F12”. For ECM, use “Fibroblast” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 3

culture “adherent” human PSC and unknown species cell in “alpha-MEM” for “2” days. For ECM, use “type IV collagen” at “0.005” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 3: endothelial 1

day 3 ~ 8

culture “adherent” human PSC and unknown species cell in “alpha-MEM” for “5” days. For ECM, use “type IV collagen” at “0.005” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “5” days.

Ideally, “EC” should be obtained at the end of culture.