Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells

DOI: 10.1038/srep22868

Authors: Li Zou; Qingshan Chen; Zachary Quanbeck; Joan E. Bechtold; Dan S. Kaufman

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “unspecified medium” for “1” days. Medium should be changed once in “1” days.

Ideally, “PSC” should express “NANOG”, “SOX2”, “POU5F1”, “SSEA4”, “Tra-1-60”, “Tra-1-81” at the end of culture.