Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells

DOI: 10.1038/srep16842

Authors: Takehiko Matsuo; Hidetoshi Masumoto; Shuhei Tajima; Takeshi Ikuno; Shiori Katayama; Kenji Minakata; Tadashi Ikeda; Kohei Yamamizu; Yasuhiko Tabata; Ryuzo Sakata; Jun K. Yamashita

Step 1: mesoderm 1

day 0 ~ 4

culture “adherent” mouse ESC in “alpha-MEM” for “4” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “mesoderm” should express “KDR” at the end of culture.

Step 2: endothelial 1

day 4 ~ 7

culture “adherent” mouse mesoderm in “alpha-MEM” for “3” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “EC” should express “PECAM1”, “VWF” at the end of culture.