Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair

DOI: 10.1038/srep11019

Authors: Shin-Jeong Lee; Changwon Park; Ji Yoon Lee; Sangsung Kim; Pil Jae Kwon; Woansang Kim; Yong Heui Jeon; Eugine Lee; Young-sup Yoon

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”.

Step 2: endothelial 1

day 1 ~ 8

culture “spheroid” human PSC in “unspecified medium” for “7” days. Medium should be changed once in “7” days.

Ideally, “LEC” should express “PROX1”, “LYVE1”, “VEGFR3”, “PODOPLANIN” at the end of culture.

Step 3: endothelial 2

day 8 ~ 22

culture “adherent” human PSC in “alpha-MEM” for “14” days. Medium should contain factors shown in the following table, and should be changed once in “14” days.

Ideally, “LEC” should express “PROX1”, “LYVE1”, “VEGFR3”, “PODOPLANIN” at the end of culture.