Novel codon-optimized mini-intronic plasmid for efficient, inexpensive and xeno-free induction of pluripotency

DOI: 10.1038/srep08081

Authors: Sebastian Diecke; Jiamiao Lu; Jaecheol Lee; Vittavat Termglinchan; Nigel G. Kooreman; Paul W. Burridge; Antje D. Ebert; Jared M. Churko; Arun Sharma; Mark A. Kay; Joseph C. Wu

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “PSC” should express “POU5F1”, “NANOG”, “SOX2”, “TRA-1-81”, “TRA-1-60” at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”.

Ideally, “EC” should be obtained at the end of culture.