Pigment Epithelium-Derived Factor Secreted from Retinal Pigment Epithelium Facilitates Apoptotic Cell Death of iPSC

DOI: 10.1038/srep02334

Authors: Hoshimi Kanemura; Masahiro J. Go; Naoki Nishishita; Noriko Sakai; Hiroyuki Kamao; Yoji Sato; Masayo Takahashi; Shin Kawamata

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “ReproFF2”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.