ICU patient-on-a-chip emulating orchestration of mast cells and cerebral organoids in neuroinflammation

DOI: 10.1038/s42003-024-07313-z

Authors: Pelin Saglam-Metiner; Sena Yanasik; Yusuf Caglar Odabasi; Jennifer Modamio; Moritz Negwer; Cigir Biray-Avci; Ayse Guler; Ali Erturk; Ender Yildirim; Ozlem Yesil-Celiktas

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Step 2: mesoderm 1

day 1 ~ 5

culture “adherent” human PSC in “mTeSR” for “4” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 3: mesoderm 2

day 5 ~ 7

culture “adherent” human PSC in “StemPro” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.