Mitochondrial permeability transition dictates mitochondrial maturation upon switch in cellular identity of hematopoietic precursors

DOI: 10.1038/s42003-024-06671-y

Authors: Sandeep P. Dumbali; Paulina D. Horton; Travis I. Moore; Pamela L. Wenzel

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse PSC in “DMEM”. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 5

culture “spheroid” mouse mesoderm in “unspecified medium” for “4” days. “mesoderm” should be “downregulated” for “Ppif”, “downregulated” for “Opa1”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “erythroid” should express “Ter-119” at the end of culture.