Sall4 and Gata4 induce cardiac fibroblast transition towards a partially multipotent state with cardiogenic potential
DOI: 10.1038/s41598-024-73975-8
Authors: Hong Gao; Saliha Pathan; Beverly R. E. A. Dixon; Aarthi Pugazenthi; Megumi Mathison; Tamer M.A. Mohamed; Todd K. Rosengart; Jianchang Yang
Step 1: endothelial 1
day 0 ~ 7
culture “unspecified method” unknown species cell in “EGM2” for “7” days. Medium should contain factors shown in the following table, and should be changed once in “7” days.
| concentration | unit | |
|---|---|---|
| FBS | 0.5 | % |
Ideally, “EC” should express “PECAM1”, “CDH5” at the end of culture.