Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

DOI: 10.1038/s41598-020-73908-1

Authors: Keisuke Sekine; Shimpei Ogawa; Syusaku Tsuzuki; Tatsuya Kobayashi; Kazuki Ikeda; Noriko Nakanishi; Kenta Takeuchi; Eriko Kanai; Yugo Otake; Satoshi Okamoto; Tsuyoshi Kobayashi; Takanori Takebe; Hideki Taniguchi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemFit AK03N”. For ECM, use “laminin 511E8” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. For ECM, use “laminin 511E8” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 3: endothelial 1

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. For ECM, use “laminin 511E8” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “EC” should express “PECAM1”, “CDH5” at the end of culture.