The histone lysine methyltransferase SETD8 regulates angiogenesis through HES-1 in human umbilical vein endothelial cells

DOI: 10.1038/s41598-020-69103-x

Authors: Dong Kyu Choi; Young Kyu Kim; Sang Wook Park; Heejin Lee; Seul Lee; Sang A. Kim; Soo Jin Kim; Junyeop Lee; Wanil Kim; Sang-Hyun Min; Ji Hoon Yu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 2: mesoderm 1

day 1 ~ 4

culture “adherent” human PSC in “Stemdiff Mesoderm induction medium” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Step 3: endothelial 1

day 4 ~ 6

culture “adherent” human PSC and human PSC in “StemPro” for “2” days. “PSC” should be “unspecified regulation” for “SETD8”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should express “PECAM1”, “CDH5” at the end of culture.

Step 4: endothelial 2

day 6 ~ 7 (duration missing in database; assumed 1 day)

culture “adherent” unknown species cell in “EGM2”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.