In vitro modelling of alveolar repair at the air-liquid interface using alveolar epithelial cells derived from human induced pluripotent stem cells

DOI: 10.1038/s41598-020-62226-1

Authors: Sander van Riet; Dennis K. Ninaber; Harald M. M. Mikkers; Teresa D. Tetley; Carolina R. Jost; Aat A. Mulder; Thijs Pasman; Danielle Baptista; André A. Poot; Roman Truckenmüller; Christine L. Mummery; Christian Freund; Robbert J. Rottier; Pieter S. Hiemstra

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “vitronectin” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.