Identification of a combination of transcription factors that synergistically increases endothelial cell barrier resistance
DOI: 10.1038/s41598-020-60688-x
Authors: Filip Roudnicky; Bo Kyoung Kim; Yanjun Lan; Roland Schmucki; Verena Küppers; Klaus Christensen; Martin Graf; Christoph Patsch; Mark Burcin; Claas Aiko Meyer; Peter D. Westenskow; Chad A. Cowan
Step 1: endothelial 1
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “unspecified method” unknown species cell in “StemPro”. Medium should contain factors shown in the following table.
| concentration | unit | |
|---|---|---|
| VEGF | 5.0e-5 | g/l |
Step 2: endothelial 2
day 1 ~ 2 (duration missing in database; assumed 1 day)
culture “unspecified method” unknown species cell in “VascuLife VEGF Endothelial Medium”. Medium should contain factors shown in the following table.
| concentration | unit | |
|---|---|---|
| FBS | 10 | % |
| Glutamine | 0.004 | mol/l |
| heparin sulfate | 750 | U/l |
| FGF | 5.0e-6 | g/l |
| EGF | 5.0e-6 | g/l |
| VEGF | 5.0e-6 | g/l |
| IGF1 | 1.5e-5 | g/l |
| hydrocortisone hemisuccinate | 0.001 | g/l |
| Ascorbic Acid | 0.05 | g/l |
| SB431542 | 1.0e-5 | mol/l |
Ideally, “EC” should be obtained at the end of culture.