Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation

DOI: 10.1038/s41598-019-53054-z

Authors: Lauren N. Randolph; Xiaoping Bao; Michael Oddo; Xiaojun Lance Lian

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “LaSR medium” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 2: Primitive streak 1

day 2 ~ 3

culture “adherent” human PSC in “LaSR medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 3: endothelial 1

day 3 ~ 6

culture “adherent” human PSC in “LaSR medium” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “EPC” should express “CDH5”, “CD34” at the end of culture.