Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells

DOI: 10.1038/s41598-019-45514-3

Authors: Giuseppe Pettinato; Sylvain Lehoux; Rajesh Ramanathan; Mohamed M. Salem; Li-Xia He; Oluwatoyosi Muse; Robert Flaumenhaft; Melissa T. Thompson; Emily A. Rouse; Richard D. Cummings; Xuejun Wen; Robert A. Fisher

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.