A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes
DOI: 10.1038/s41598-018-31848-x
Authors: Fabio Maiullari; Marco Costantini; Marika Milan; Valentina Pace; Maila Chirivì; Silvia Maiullari; Alberto Rainer; Denisa Baci; Hany El-Sayed Marei; Dror Seliktar; Cesare Gargioli; Claudia Bearzi; Roberto Rizzi
Step 1: iPSC/ESC maintainance
day 0 ~ 1
culture “adherent” mouse PSC in “KnockOut DMEM” for “1” days. For ECM, use “Fibroblast” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.
| concentration | unit | |
|---|---|---|
| KSR | 20 | % |
| Glutamine | 0.002 | mol/l |
| NEAA | 1.0e-4 | mol/l |
| FGF | 1.0e-5 | g/l |
| VPA | 5.0e-4 | mol/l |
| 2-mercaptoethanol | 1.0e-4 | mol/l |
| LIF | 1.0e+6 | U/l |
| PSA | 100000 | U/l |
| streptomycin | 100 | g/l |
Ideally, “PSC” should express “POU5F1”, “SOX2”, “NANOG”, “SSEA1” at the end of culture.