Combinatorial Extracellular Matrix Microenvironments for Probing Endothelial Differentiation of Human Pluripotent Stem Cells

DOI: 10.1038/s41598-017-06986-3

Authors: Luqia Hou; Joseph J. Kim; Maureen Wanjare; Bhagat Patlolla; John Coller; Vanita Natu; Trevor J. Hastie; Ngan F. Huang

Step 1: endothelial 1

day 0 ~ 2

culture “device” human PSC in “alpha-MEM” for “2” days. For ECM, use “collagen IV+heparan sulfate+laminin” at “0.5” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should express “PECAM1”, “CDH5”, “KDR”, “integrin β3”, “CD105” at the end of culture.

Step 2: endothelial 2

day 2 ~ 5

culture “device” human PSC in “alpha-MEM” for “3” days. For ECM, use “collagen IV+heparan sulfate+laminin” at “0.5” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “EC” should express “PECAM1”, “CDH5”, “CD105”, “KDR” at the end of culture.