A comprehensive library of human transcription factors for cell fate engineering

DOI: 10.1038/s41587-020-0742-6

Authors: Alex H. M. Ng; Parastoo Khoshakhlagh; Jesus Eduardo Rojo Arias; Giovanni Pasquini; Kai Wang; Anka Swiersy; Seth L. Shipman; Evan Appleton; Kiavash Kiaee; Richie E. Kohman; Andyna Vernet; Matthew Dysart; Kathleen Leeper; Wren Saylor; Jeremy Y. Huang; Amanda Graveline; Jussi Taipale; David E. Hill; Marc Vidal; Juan M. Melero-Martin; Volker Busskamp; George M. Church

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2

culture “adherent” human PSC in “mTeSR” for “1” days. “PSC” should be “upregulated” for “ETV2”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “EC” should express “PLVAP”, “PECAM1”, “CDH5” at the end of culture.