A prenatal skin atlas reveals immune regulation of human skin morphogenesis

DOI: 10.1038/s41586-024-08002-x

Authors: Nusayhah Hudaa Gopee; Elena Winheim; Bayanne Olabi; Chloe Admane; April Rose Foster; Ni Huang; Rachel A. Botting; Fereshteh Torabi; Dinithi Sumanaweera; Anh Phuong Le; Jin Kim; Luca Verger; Emily Stephenson; Diana Adão; Clarisse Ganier; Kelly Y. Gim; Sara A. Serdy; CiCi Deakin; Issac Goh; Lloyd Steele; Karl Annusver; Mohi-Uddin Miah; Win Min Tun; Pejvak Moghimi; Kwasi Amoako Kwakwa; Tong Li; Daniela Basurto Lozada; Ben Rumney; Catherine L. Tudor; Kenny Roberts; Nana-Jane Chipampe; Keval Sidhpura; Justin Englebert; Laura Jardine; Gary Reynolds; Antony Rose; Vicky Rowe; Sophie Pritchard; Ilaria Mulas; James Fletcher; Dorin-Mirel Popescu; Elizabeth Poyner; Anna Dubois; Alyson Guy; Andrew Filby; Steven Lisgo; Roger A. Barker; Ian A. Glass; Jong-Eun Park; Roser Vento-Tormo; Marina Tsvetomilova Nikolova; Peng He; John E. G. Lawrence; Josh Moore; Stephane Ballereau; Christine B. Hale; Vijaya Shanmugiah; David Horsfall; Neil Rajan; John A. McGrath; Edel A. O’Toole; Barbara Treutlein; Omer Bayraktar; Maria Kasper; Fränze Progatzky; Pavel Mazin; Jiyoon Lee; Laure Gambardella; Karl R. Koehler; Sarah A. Teichmann; Muzlifah Haniffa

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” unknown species cell and human PSC in “unspecified medium”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “EC” should be obtained at the end of culture.