Versatile synthetic alternatives to Matrigel for vascular toxicity screening and stem cell expansion

DOI: 10.1038/s41551-017-0096

Authors: Eric H. Nguyen; William T. Daly; Ngoc Nhi T. Le; Mitra Farnoodian; David G. Belair; Michael P. Schwartz; Connie S. Lebakken; Gene E. Ananiev; Mohammad Ali Saghiri; Thomas B. Knudsen; Nader Sheibani; William L. Murphy

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “E8” for “1” days. For ECM, use “hydrogel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “ESC” should express “NANOG”, “OCT3/4”, “SOX-2” at the end of culture.