Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip

DOI: 10.1038/s41551-017-0069

Authors: Samira Musah; Akiko Mammoto; Thomas C. Ferrante; Sauveur S. F. Jeanty; Mariko Hirano-Kobayashi; Tadanori Mammoto; Kristen Roberts; Seyoon Chung; Richard Novak; Miles Ingram; Tohid Fatanat-Didar; Sandeep Koshy; James C. Weaver; George M. Church; Donald E. Ingber

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Step 2: mesoderm 1

day 1 ~ 3

culture “adherent” human PSC in “DMEM/F12” for “2” days. For ECM, use “laminin-511 E8” at “0.005” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “mesoderm” should express “goosecoid”, “HAND1”, “brachyury” at the end of culture.

Step 3: mesoderm 2

day 3 ~ 17

culture “adherent” human mesoderm in “DMEM/F12” for “14” days. For ECM, use “laminin-511 E8” at “0.005” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “14” days.

Ideally, “IM” should express “OSR1”, “WT1”, “PAX2” at the end of culture.