Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions

DOI: 10.1038/s41551-016-0003

Authors: Xiaoping Bao; Xiaojun Lian; Timothy A. Hacker; Eric G. Schmuck; Tongcheng Qian; Vijesh J. Bhute; Tianxiao Han; Mengxuan Shi; Lauren Drowley; Alleyn T. Plowright; Qing-Dong Wang; Marie-Jose Goumans; Sean P. Palecek

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2

culture “adherent” human PSC in “RPMI” for “1” days. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PS” should be obtained at the end of culture.

Step 3: mesoderm 1

day 2 ~ 4

culture “adherent” human PS in “RPMI” for “2” days. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “mesoderm” should be obtained at the end of culture.