Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vessel

DOI: 10.1038/s41467-024-51487-3

Authors: Erica Carolina; Yoshiki Kuse; Ayumu Okumura; Kenji Aoshima; Tomomi Tadokoro; Shinya Matsumoto; Eriko Kanai; Takashi Okumura; Toshiharu Kasai; Souichiro Yamabe; Yuji Nishikawa; Kiyoshi Yamaguchi; Yoichi Furukawa; Naoki Tanimizu; Hideki Taniguchi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemFit AK02N”. For ECM, use “Laminin-511E8” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “EC” should express “PECAM1”, “TGFB1” at the end of culture.