Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model

DOI: 10.1038/s41467-024-48663-w

Authors: Sudip Kumar Paul; Motohiko Oshima; Ashwini Patil; Masamitsu Sone; Hisaya Kato; Yoshiro Maezawa; Hiyori Kaneko; Masaki Fukuyo; Bahityar Rahmutulla; Yasuo Ouchi; Kyoko Tsujimura; Mahito Nakanishi; Atsushi Kaneda; Atsushi Iwama; Koutaro Yokote; Koji Eto; Naoya Takayama

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “AK02N medium”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 4

culture “adherent” human PSC in “unspecified medium” for “3” days. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “PS” should be obtained at the end of culture.

Step 3: endothelial 1

day 4 ~ 11

culture “adherent” human VPC in “unspecified medium” for “7” days. Medium should contain factors shown in the following table, and should be changed once in “7” days.

Ideally, “EC” should express “CDH5”, “KDR”, “CD34”, “PECAM1”, “VWF”, “NOS3” at the end of culture.