Epigenetic landscape reveals MECOM as an endothelial lineage regulator

DOI: 10.1038/s41467-023-38002-w

Authors: Jie Lv; Shu Meng; Qilin Gu; Rongbin Zheng; Xinlei Gao; Jun-dae Kim; Min Chen; Bo Xia; Yihan Zuo; Sen Zhu; Dongyu Zhao; Yanqiang Li; Guangyu Wang; Xin Wang; Qingshu Meng; Qi Cao; John P. Cooke; Longhou Fang; Kaifu Chen; Lili Zhang

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Step 2: mesoderm 1

day 1 ~ 3

culture “adherent” human PSC in “DMEM/F12” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 3: endothelial 1

day 3 ~ 5

culture “adherent” human PSC in “EGM2” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should express “MECOM”, “KDR”, “CDH5”, “VWF”, “ERG”, “DLL4”, “EFNB2”, “NOS3”, “NR2F2” at the end of culture.